rabbit polyclonal antibody to collageniv  (Abcam)

Journal: IBRO Neuroscience Reports

Article Title: Early mobilization in spinal cord injury promotes changes in microglial dynamics and recovery of motor function

doi: 10.1016/j.ibneur.2022.04.002

Figure Lengend Snippet: A, B: Merged Images of quadruple staining with GFAP (green), GAP43 (white), and collagenⅣ (red) at the spinal cord injury lesion. Image of the 3-week EM group spinal cord lesion site overview is shown in A. Scale bar: 200 µm. The lesion center is enclosed by the white dotted line. B: Enlargement image of spinal cord lesion. Yellow arrows indicate representative GAP43 structures (white). The border of lesion center is indicated by the yellow dotted line. Scale bars: 50 µm. C : Comparison of the area of GAP43 structures and lesion center between the 3-week NEM and 3-week EM groups. Data are presented as mean ± SEM. * p-value= 0.011. D : Comparison of the GAP43 density of lesion center between the 3-week NEM and 3-week EM groups. Data are presented as mean ± SEM. * p-value= 0.016.

Article Snippet: Mouse monoclonal antibodies against GAP43 (1:500; ab12990, Abcam), rabbit polyclonal antibodies against collagenIV (1:100; ab6586, Abcam), and goat polyclonal antibodies against glial fibrillary acidic protein (GFAP; 1:100; ab53554, Abcam) were used as primary antibodies to label growth cones, collagen fibers, and astrocytes, respectively.

Techniques: Staining, Comparison

Journal: Journal of Cerebral Blood Flow & Metabolism

Article Title: Region-specific permeability of the blood–brain barrier upon pericyte loss

doi: 10.1177/0271678X17697340

Figure Lengend Snippet: List of validated antibodies for immunofluorescence staining of brain sections.

Article Snippet: Basal lamina (CollagenIV) , Rabbit polyclonal, serotec Goat polyclonal, BioRad.

Techniques: Immunofluorescence, Staining, Marker